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Volume 22, Issue 2 (4-2025)
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Kadkhoda P, Rezaei Farimani A, Chamani E, Hashemi S M. Molecular Mechanisms of HT-29 Colorectal Cancer Cell Death Induced by Artemisia annua Methanolic Extract. Mod Care J 2025; 22 (2) : e148090
URL: http://mcj.bums.ac.ir/article-1-326-en.html
URL: http://mcj.bums.ac.ir/article-1-326-en.html
1- Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran
2- Department of Biochemistry, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran
3- Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran ,hashemi.smhm76@gmail.com
2- Department of Biochemistry, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran
3- Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran ,
Abstract: (160 Views)
Background:Although chemotherapy and targeted therapies have improved survival outcomes, there is growing interest in incorporating herbal medicines, such as Artemisia annua, into cancer treatment.
Objectives:This study aims to investigate the mechanisms underlying the effects of A. annua methanolic extract on HT-29 colorectal cancer cells, particularly focusing on cell viability and the modulation of p53 and BAX/Bcl2 ratio expression.
Methods:The half-maximal inhibitory concentration (IC50) of A. annua methanolic extract in HT-29 cells was determined via the MTT viability assay, while apoptosis was assessed using the propidium iodide (PI) staining method. Reverse transcription (RT)-PCR was performed to evaluate gene expression in the HT-29 cell line. Values are presented as the average of three separate determinations and expressed as mean ± SD.
Results:The results showed that the viability of HT-29 cells significantly decreased in a dose- and time-dependent manner (IC50 for 48 h: 1358.1 μg.mL-1). Propidium iodide staining results confirmed the findings of the MTT assay. Gene expression analysis revealed a decrease in p53 expression and a two-fold increase in the BAX/Bcl2 ratio following exposure to 1000 μg.mL-1 of A. annua extract.
Conclusions:The findings from the MTT and PI assays confirm the cytotoxic effects of A. annua extract on HT-29 cells. However, the gene expression analysis of BAX, Bcl2, and p53 suggests that the observed cell death is not primarily due to apoptosis.
Objectives:This study aims to investigate the mechanisms underlying the effects of A. annua methanolic extract on HT-29 colorectal cancer cells, particularly focusing on cell viability and the modulation of p53 and BAX/Bcl2 ratio expression.
Methods:The half-maximal inhibitory concentration (IC50) of A. annua methanolic extract in HT-29 cells was determined via the MTT viability assay, while apoptosis was assessed using the propidium iodide (PI) staining method. Reverse transcription (RT)-PCR was performed to evaluate gene expression in the HT-29 cell line. Values are presented as the average of three separate determinations and expressed as mean ± SD.
Results:The results showed that the viability of HT-29 cells significantly decreased in a dose- and time-dependent manner (IC50 for 48 h: 1358.1 μg.mL-1). Propidium iodide staining results confirmed the findings of the MTT assay. Gene expression analysis revealed a decrease in p53 expression and a two-fold increase in the BAX/Bcl2 ratio following exposure to 1000 μg.mL-1 of A. annua extract.
Conclusions:The findings from the MTT and PI assays confirm the cytotoxic effects of A. annua extract on HT-29 cells. However, the gene expression analysis of BAX, Bcl2, and p53 suggests that the observed cell death is not primarily due to apoptosis.
Article number: e148090
Article Types: Research Article |
Subject:
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Received: 2024/04/23 | Accepted: 2024/08/13 | Published: 2025/04/15
Received: 2024/04/23 | Accepted: 2024/08/13 | Published: 2025/04/15
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