en General پژوهشي Research Article Background and Aim: Blood transfusion can save a million lives. Nonetheless, every recipient is at risk for transfusion-transmissible infection, chiefly hepatitis B infection. The aim of this study was to compare stationary and mobile blood donation centers respecting the prevalence of hepatitis B in 2007 - 2016. Methods: This cross-sectional descriptive study compared hepatitis B prevalence in stationary and mobile blood donation centers in Southern Khorasan in a ten-year period from 2007 to 2016. Data were retrieved from data sheets available from Iranian blood transfusion organization. Results: A total of 157063 blood units had been collected in 2007 - 2016. The number of blood units collected by stationary and mobile centers was 129,899 (82.7%) and 27,164 (17.3%), respectively. Total hepatitis B prevalence rate was 0.22% (348 cases). This rate in stationary and mobile centers was 0.18% (232 cases) and 0.40% (116 cases), respectively. Hepatitis B prevalence was significantly greater in mobile centers than in stationary centers (P < 0.001). Moreover, hepatitis B prevalence in the second five years, i.e. 2012 - 2016, was significantly lower than that in the first five years, i.e. 2007 - 2011 (P < 0.001). Conclusions: Hepatitis B prevalence in mobile blood donation centers is significantly higher than that in stationary centers. This problem can be due to the limited health-related knowledge of blood donors in rural areas where mainly mobile centers collect blood units. Therefore, restricting blood collection by mobile centers may be indicated until necessary health- and donation-related educations are provided to all people in high-risk areas. Blood Transfusion,Hepatitis B Virus Infection,Stationary Blood Donation Center,Mobile Blood Donation Center 0 0 http://mcj.bums.ac.ir/browse.php?a_code=A-10-1-62&slc_lang=en&sid=1 Mohammadreza Ameli 10031947532846003558 10031947532846003558 No Iranian Blood Transfusion Organization Research Center, South-Khorasan Blood Transfusion Service, Birjand, IR Iran Mahsa Najafzadeh 10031947532846003559 10031947532846003559 No Student Research Committee, Birjand University of Medical Sciences, Birjand, IR Iran Seyed Mehdi Sajjadi 10031947532846003560 10031947532846003560 Yes Seyed Mehdi Sajjadi, Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, IR Iran. E-mail: mehdi.sadjadi@bums.ac.ir