Modern Care Journal

en Prevalence, Antifungal Susceptibility, and Biofilm-Forming Ability of Candida Species from Clinical Samples General پژوهشي Research Article <div style="text-align: justify;">Introduction: Candida species, as opportunistic fungal pathogens, can form biofilms on biotic and abiotic surfaces, leading to resistance to current antifungals used in the treatment of candidiasis. This study aimed to identify Candida species from clinical samples and to assess antifungal susceptibility patterns and biofilm-forming ability. Objective: This study aimed to identify different Candida species from clinical samples and to assess the antifungal susceptibility patterns and biofilm formation ability. Methods: In this study, 100 Candida isolates were obtained from various clinical samples (blood, urine, body fluids, etc.) by culturing. Antifungal susceptibility assays for Candida isolates were performed using the broth microdilution technique in accordance with the Clinical and Laboratory Standards Institute M27-A3/S4 guidelines. Biofilm formation was measured by the colorimetric microtiter method. Results: Candida albicans (48%) was the most common Candida species identified, followed by C. glabrata (34%), C. dubliniensis (9%), C. tropicalis (5%), C. parapsilosis (1%), C. krusei (1%), and two mixed colonies were identified. The antifungal susceptibility pattern shows that Candida isolates exhibit varying susceptibilities. C. albicans was 100% susceptible to amphotericin B, whereas C. glabrata was 70.58% resistant to amphotericin B. The Candida parapsilopsis isolate was susceptible to all antifungals. According to the results, Candida isolates have a moderate ability to form biofilms. Conclusion: Therefore, periodic monitoring of Candida species prevalence and antifungal susceptibility patterns necessitates selecting the appropriate therapeutic protocol, as well as the development of novel antifungal compounds and treatment strategies.</div> Antifungal, Biofilm formation, Candidiasis, Drug resistance 18 25 http://mcj.bums.ac.ir/browse.php?a_code=A-10-30-1&slc_lang=en&sid=1 Mahdi Mohammadi mahdi.zibad@gmail.com 1 0009-0003-0382-1373 No Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran. Mohammad Hassan Namaei mhnamaei@gmail.com 2 10031947532846006228 No Department of Laboratory Sciences, Faculty of Paramedical and Rehabilitation Sciences, Mashhad University of Medical Sciences, Mashhad, Iran. Mostafa Ashrafipour mostafaashrafipour@yahoo.com 3 10031947532846006229 No Department of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashahad, Iran. Mohammad Amin Abdolahi Ziaaldini abdolahi.maz@gmail.com 4 0009-0001-4709-007X No Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran. Fatemeh Nikoomanesh fateme.nikoomanesh@bums.ac.ir 5 10031947532846006231 Yes Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran.